Schalke's preparations for the new season suffered a setback as they lost 2-1 to second division side Union Berlin in a friendly.
Vicente Sanchez was on target for the Royal Blues in the defeat and manager Felix Magath criticised his side's timid approach.
They travel to face Germania Windeck in the first round of the DFB-Pokal next Saturday.
"We were not lively enough in the middle," said Magath on the club's website. "I would have liked more tenacity."
Another Bundesliga side, Hannover fell to a 2-1 defeat at sixth-tier side Anker Wismar.
Arnold Bruggink scored the consolation for Dieter Hecking's side, who are still searching for form ahead of their trip to Trier next weekend.
Meanwhile, Stuttgart impressed with a 5-0 win over second division side Greuther Furth.
With their search for a new striker to replace Mario Gomez continuing, Cipriana Marica needed just seconds to stake his claim for a place in the attack of Markus Babbel's side.
Cacau with two, Martin Lanig and Julian Schieber scored the other goals for the Swabians.
Hertha Berlin and Borussia Monchengladbach were held to draws by St Pauli and Barclays Premier League side Bolton respectively, while Borussia Dortmund managed a win and a defeat in a mini-tournament staged at their Westfalenstadion.
After losing on a penalty shoot-out to tournament winners Valencia, they defeated Udinese 2-1 with goals from Mohamed Zidan and Kevin Grosskreutz.
Eintracht Frankfurt saw off Kaiserslautern 2-0, but there was a 1-0 defeat for Bochum against Turkish first division side Trabzonspor.
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